Everything about Thermocycler totally explained
The
Thermal Cycler (also known as a
Thermocycler,
PCR Machine or
DNA Amplifier) is a
laboratory apparatus used to amplify segments of
DNA via the
polymerase chain reaction (PCR) process. The device has a
thermal block with holes where tubes holding the
PCR reaction mixtures can be inserted. The cycler then raises and lowers the temperature of the block in discrete, pre-programmed steps.
The earliest Thermal Cyclers were designed for use with the
Klenow fragment of
DNA Polymerase I. Since this enzyme is destroyed during each heating step of the amplification process, new enzyme had to be added every cycle. This led to a cumbersome machine based on an automated
pipettor, with open reaction tubes (see photo). Later, the PCR process was adapted to the use of thermostable
DNA polymerase from
Thermus aquaticus, which greatly simplified the design of the Thermal Cycler.
Modern Thermal Cyclers are often equipped with a
hot bonnet, a heated plate that presses against the lids of the reaction tubes. This prevents condensation of water from the reaction mixtures to the insides of the lids and makes it unnecessary to use PCR oil. Some thermal cyclers are equipped with multiple blocks allowing several different
PCR reactions to be carried out simultaneously. Also some apparatus have a
gradient function, which allows different temperatures in different parts of the block. This is particularly useful when testing suitable annealing temperatures for
primers. The prices for thermal cyclers start from
USD $2500 (in
2004).
Further Information
Get more info on 'Thermocycler'.
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